Identification and characterization of CAT1 gene during drought stress in moth bean [Vigna aconitifolia (Jacq.) Marechal]

Authors

  • Manoj Kumar Meena
  • Ishani Dogra
  • Yemmanur Sudarsan
  • Anil Kumar Sharma
  • Dhurendra Singh
  • Vikas Sharma
  • Chet Ram ICAR-Central Institute for Arid Horticulture, Bikaner

DOI:

https://doi.org/10.53911/JAE.2022.14224

Keywords:

Bioinformatics analysis, CAT1 gene, Drought stress, Gene expression, Sequence validation

Abstract

Moth bean [Vigna aconitifolia (Jacq.) Marechal] is a drought hardy orphan pulse crop. However, the information related to the identification and characterization of drought stress tolerance genes is very limited. Therefore, the present investigation was formulated to identified and characterized drought tolerant gene(s) from moth bean. Five genes were selected from available expression studies of moth bean and their expression pattern was assessed during time course experiment of drought stress in moth bean. During the time course experiment of drought stress in moth bean, the catalase1 (CAT1) gene was exponentially expressed in up-regulated manner. Thus, CAT1 gene of moth bean was identified as potential candidate gene and validated through Sanger’s sequencing. The genomic sequence CAT1 gene was named as VacoCAT1 and was further characterized using various bioinformatics tools. The VacoCAT1 showed an ORF with incomplete length of 213 bp which encoded 71 amino acids. The coding sequence of VacoCAT1 gene was shown a single exon due to incomplete nature of genomic sequences. The multiple sequence alignment of VacoCAT1 revealed the highly conserved region at 3’ site of the gene as compared to CAT1 gene of other crop species including legumes. The phylogenetic analysis of VacoCAT1 and CAT1 gene of other crop species including legumes revealed three clusters. The cluster VacoCAT1 gene showed close proximity with V. radiata CAT1 in cluster one of phylogenetic tree. The identified and characterized VacoCAT1 gene can be utilized as a genomic resource for enhance drought tolerance in susceptible pulses as well as other crops.

Downloads

Download data is not yet available.

References

Bhati TK, Shalander K, Amare H & Whitbread AM. 2017. Assessment of agricultural technologies for dryland systems in South Asia. Monograph. ICRISAT, Patancheru, Hyderabad, pp - 68.

Soni P, Rizwan M, Bhatt KV, Mohapatra T & Singh G. 2011. In-vitro response of Vigna aconitifolia to drought stress induced by PEG–6000. Journal of Stress Physiology and Biochemistry, 7:108-121.

Tiwari B, Kalim S, Bangar P, Kumar S, Kumari R, Barman P & Bhat, K.V. (2017). Expression analysis in response to heat stress in moth bean (Vigna aconitifolia (Jacq.) Marechal). Electronic Journal of Plant Breeding 8: 1293-1297.

Tiwari B, Kalim S, Tyagi N, Kumari R, Bangar P, Barman P, Kumar S, Gaikwad A & Bhat KV. 2018. Identification of genes associated with stress tolerance in moth bean [Vigna aconitifolia (Jacq.) Marechal], a stress-hardy crop. Physiology and Molecular Biology of Plants, 24:551–561.

Lisar SYS, Motafakkerazad R, Hossain MM & Rahman IMM. 2012. Water stress in plants: Causes, effects and responses. In Ismile, Rahman, M. and Hasegawa, H. (Eds.), Water stress in plants (pp. 1-15). IntechOpen.

Farooq M, Wahid A, Kobayashi N, Fujita D & Basra SMA. 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development, 29:185-212.

Duan B, Yang Y, Lu Y, Korpelainen H, Berninger F & Li C. 2007. Interactions between drought stress, ABA and genotypes in Picea asperata. Journal of Experimental Botany, 58:3025-3036.

Chaves MM, Flexas J & Pinheiro C. 2009. Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany, 103:551-560.

Ashraf M. 2009. Inducing drought tolerance in plants. Biotechnology Advances, 28:169-183.

Govind G, Gowda HVT, Kalaiarasi PJ, Iyer DR, Muthappa SK, Nese S & Makarla UK. 2009. Identification and functional validation of a unique set of drought-induced genes preferentially expressed in response to gradual water stress in peanut. Molecular Genetics and Genomic, 281:591-605.

Kumar A, Sharma S, Chunduri V, Kaur A, Kaur S, Malhotra M, Kumar A, Kapoor P, Kumari A, Kaur J, Sonah H & Garg M. 2020. Genome-wide identification and characterization of heat shock protein family reveal role in development and stress conditions in Triticum aestivum L. Scientific Reports, 10:7858.

Gurjar K, Rampuria S, Joshi U, Palit P, Bhatt K V, Mohapatra T & Sharma R. 2014. Identification of heat-related ESTs in mothbean through suppression subtraction hybridization. Applied biochemistry and biotechnology, 173:2116-2128.

Rampuria S, Joshi U, Palit P, Deokar AA, Meghwal RR, Mohapatra T, Srinivasan R, Bhatt KV & Sharma R. 2012. Construction and analysis of an SSH cDNA library of early heat-induced genes of Vigna aconitifolia variety RMO-40. Genome 55: 783-96.

Harsh A, Sharma YK, Joshi U, Rampuria S, Singh G, Kumar S & Sharma R. 2016. Effect of short-term heat stress on total sugars, proline and some antioxidant enzymes in moth bean (Vigna aconitifolia). Annals of Agricultural Sciences, 61:57-64.

Priya A M, Krishnan S R & Ramesh M. 2015. Ploidy stability of Oryza sativa. L cv. IR64 transformed with the moth bean P5CS gene with significant tolerance against drought and salinity. Turkish Journal of Biology, 39: 407-416.

Sharma R, Jain M, Kumar S & Kumar V. 2014. Evaluation of differences among Vigna aconitifolia varieties for acquired t tolerance. Agricultural Research, 3: 104-112.

Ortiz C & Cardemil L. 2001. Heat‐shock responses in two leguminous plants: A comparative study. Journal of Experimental Botany, 52: 1711–1719.

Wang K, Zhang X, Goatley M & Ervin E. 2014. Heat shock proteins in relation to heat stress tolerance of creeping bentgrass at different N levels. PLoS One, 9: e102914.

Yong B, Wang X, Xu P, Zheng H, Fei X, Hong Z, Ma Q, Miao Y, Yuan X, Jiang Y & Shao H. 2017. Isolation and abiotic stress resistance analyses of a catalase gene from Ipomoea batatas (L.) Lam. BioMed Research International, 2017: 10.

Wang W, Cheng Y, Chen D, Liu D, Hu M, Dong J, Zhang X, Song L & Shen F. 2019. The catalase gene family in cotton: genome-wide characterization and bioinformatics analysis. Cells, 8(2): 86.

Koonin EV & Galperin MY. 2003. Principles and Methods of Sequence Analysis. In Boston (Ed.), Sequence - Evolution - Function: Computational approaches in comparative genomics, Kluwer Academic, 2003.

Koramutla MK, Ram C, Bhatt D, Annamalai M & Bhattacharya R. 2019. Genome-wide identification and expression analysis of sucrose synthase genes in allotetraploid Brassica juncea. Gene, 707: 126-135.

Polidoros AN, Mylona PV, Pasentsis K & Tsaftaris AS. 2003. Catalase expression in normal metabolism and under stress in the model legume Medicago truncatula. In Proceedings of International congress on Genes, gene families and isozymes, (pp. 147–156), Medimond Srl, Berlin.

Wu X, Li J, Tan J & Liu X. 2016. Molecular cloning, characterization and expression analysis of a catalase gene in Paphia textile. Acta Oceanologica Sinica, 35: 65–73.

Sharma S & Hooda V. 2018. Identification of coding sequence and its use for functional and structural characterization of catalase from Phyllanthus emblica. Bioinformation, 14: 8-14.

Klotz MG & Loewen PC. 2003. The molecular evolution of catalatic hydroperoxidases: evidence for multiple lateral transfer of genes between prokaryota and from bacteria into eukaryote. Molecular Biology and Evolution, 20:1098–1112.

Figueroa-Yanez L, Cano-Sosa J, Castano E, Arroyo-Herrera A L, Caamal-Velazquez J H, Sanchez-Teyer F, Lopez-Gomez R, Santos-Briones C D L & Rodrıguez-Zapata L. 2012. Phylogenetic relationships and expression in response to low temperature of a catalase gene in banana (Musa acuminate cv. ‘‘Grand Nain’’) fruit. Plant Cell Tissue and Organ Culture, 109:429-438.

Muthusamy SK, Sivalingam PN, Sridhar J, Singh D and Haldhar SM. 2017. Biotic stress inducible promoters in crop plants-a review. Journal of Agriculture and Ecology 4: 14-24.

Downloads

Published

2022-12-15

How to Cite

Manoj Kumar Meena, Ishani Dogra, Yemmanur Sudarsan, Anil Kumar Sharma, Dhurendra Singh, Vikas Sharma, & Chet Ram. (2022). Identification and characterization of CAT1 gene during drought stress in moth bean [Vigna aconitifolia (Jacq.) Marechal]. Journal of Agriculture and Ecology, 14(14), 164–172. https://doi.org/10.53911/JAE.2022.14224