Zinc solubilizing plant growth promoting microbes produce zinc nanoparticles
Keywords:Zinc Nano Particles, Pseudomonas, Bacillus, Azospirillum, FTIR, Zeta Potential, ZnO, ZnCO3
Strains of Pseudomonas, Bacillus, and Azospirillum with plant growth promoting ability were checked for their zinc solubilizing ability at ICAR-CRIDA, Hyderabad, India. Efficient zinc solubilizing microorganisms were evaluated for their ability to produce nano-scale zinc particles. The nanoparticles from the cell-free culture filtrates obtained from these strains were characterized for particle size, Zeta potential and functional groups. Presence of Zn nanoparticles in the bacterial culture filtrate was confirmed by particle distribution and Scanning electron microscope (SEM) analysis. Most properties of nanoparticles are size dependent. Zinc nanoparticles were observed to be spherical in shape and size ranged from 52.0 to 106.0nm. Zeta potential of the Zn nanoparticles was estimated to understand the stability of the particles. The measured zeta potentials varied from -14.5mV to +179.10 mV indicating high stability and dispersion of the zinc nanoparticles. FTIR peaks at different wave numbers depicted the role of functional groups of proteins in the biosynthesis of Zn nanoparticles. These results demonstrate the green synthesis of zinc nanoparticles by the plant growth promoting and zinc solubilizing strains of Azospirillum, Pseudomonas and Bacillus.
Bhupinder S, Senthil AN, Singh BK & Usha K. 2005. Improving zinc efficiency of cereals under zinc deficiency. Current Science, 88:36-44.
Domingos RF, Tufenkji N & Wilkinson KI. 2009. Aggregation of titanium dioxide nanoparticles: role of a fulvic acid. Environmental Science Technology, 43:1282–6.
Gurunathan S, Kalishwaralal K, Vaidyanathan R, Venkataraman D, Pandian SRK, Muniyandi J, Hariharan N & Eom SH. 2009. Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli. Colloids Surf B, 741:328–35.
Guzman KAD, Finnegan MP & Banfield JF. 2006. Influence of surface potential on aggregation and transport of Titania nanoparticles. Environmental Science Technology, 24:7688-7693.
Honary S, Gharaei-Fathabad E, Paji ZK & Eslamifar, M. 2010. A Novel Biological Synthesis of Gold Nanoparticle by Enterobacteriaceae Family. Tropical Journal of Pharmacology Research, 6:887-891.
Klaus T, Joerger R, Olsson E & Granqvist CG. 1999. Silver based crystalline nanoparticles, microbially fabricated. Proceeding of National Academy Science USA, 96:13611–13614.
Minaeian S, Shahverdi AR, Nohi AS & Shahverdi HR. 2008. Extracellular biosynthesis of silver nanoparticles by some bacteria. J Sci IAU, 17:1-4.
Mortvedt JJ. 1992. Crop response to level of water-soluble zinc in granular zinc fertilizers. Fertilizer Research, 33:249–55.
Nagarajan S & Kuppusamy KA. 2011. Extracellular synthesis of zinc oxide nanoparticle using seaweeds of gulf of Mannar, India. Journal of Nanobiotechnology, 11:39-50.
Nair B & Pradeep T. 2002. Coalescence of nanoclusters and formation of submicron crystallites assisted by Lactobacillus strains. Cryst Growth Des, 2:293-98.
Prasad TNVKV, Sudhakar Y, Sreenivasulu P, Latha V, Munaswamy K, Raja Reddy TS, Sreeprasad PR, Sajanlal & Pradeep T. 2012. Effect of nanoscale zinc oxide particles on the germination, growth and yield of peanut. Journal of Plant Nutrition, 6:905-927.
Saifuddin N, Wong CW, & Nuryasumira AA. 2009. Rapid biosynthesis of silver nanoparticles using culture supernatant of bacteria with microwave irradiation. E J Chem, 6:61-70.
Singh AL. 1999. Mineral nutrition of groundnut. In: Hemantharajan A, editor. Advances in Plant Physiology (pp 161–200). Jodhpur, India: Scientific Publications.
Singh MV. 2009. Micronutrient nutritional problems in soils of India and Improvement for human and animal health. Indian Journal of Fertilizers, 5:11-26.
Sonika K, Jigmet L, Sumit S & Haq Nawaz Sheikh. 2015. Green hydrothermal synthesis and optical properties of c-Gd2S3 nanoparticles. Applied Nan science, DOI 10.1007/s13204-015-0478-7.
Suseelendra Desai, Praveen Kumar G, Uzma Sultana, Sravani Pinisetty, Mir Hassan Ahmed SK, Leo Daniel Amalraj E & Gopal Reddy. 2012. Potential microbial candidate strains for management of nutrient requirements of crops. African Journal of Microbiological Research, 6: 3924-3931
Vielkind M, Kampen I & Kwade A. 2013. Zinc Oxide Nanoparticles in bacterial growth medium Optimized dispersion and growth Inhibition of Pseudomonas putida. Adv Nanoparticles, 2:287-293.
Zhang H, Li Q, Lu Y, Sun D, Lin X & Deng X. Biosorption and bioreduction of diaminesilver complex by Corynebacterium. J Chem Technol Biotechnol, 80: 285–290.