Ascertaining hybridity of progenies in mango (Mangifera indica L.) using microsatellite (SSR) markers
Keywords:
Ascertaining hybridity, mango, microsatellite markersAbstract
In the present study (2012-13& 2013-14) seven mango genotypes viz., Amrapali, Dushehari, Janardan Pasand, Neelam, Pusa Arunima, Sensation and Tommy Atkins were used for obtaining hybrid plantlets. Artificial hybridization was attempted using Amrapali as female and Dushehari, Janardan Pasand, Neelam, Pusa Arunima, Sensation and Tommy Atkins as male donor parent and the recovery of the hybrids was very low. Fourty-two microsatellite markers (SSRs) among seven mango parental genotypes were validated against mango plantlets obtained from the crosses Amrapali x Dashehari, Amrapali x Janardan Pasand, Amrapali x Neelam, Amrapali x Pusa Arunima, Amrapali x Sensation and Amrapali x Tommy Atkins. Fourty- two primer pairs were screened, 13 primer pairs (LMMA 2, 3, 4, 7, 8, 9, 11, 12, 16 and ESTD 1, 2, 9 and 10) were found polymorphic and utilized for ascertaining hybridity of progenies obtained from seven mango parental genotypes. Out of 13 polymorphic primers, only 3 primer pairs (LMMA 11, ESTD 9 and ESTD 10) could confirm the hybridity of progenies in different cross combinations.
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References
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