Screening and identification of Piper species as rootstocks resistance against the root knot nematode under glasshouse condition
Keywords:Piper species, screening, Meloidogyne incognita, resistance
A study was conducted to evaluate the reaction of different Piper species to root knot nematode (Meloidogyne incognita) and the nematode reproduction were studied in the nematode infested pot culture experiment. Three Piper species viz., Piper colubrinum, Piper argyrophyllum and cultivated Piper nigrum varieties of IISR Sakthi, IISR Thevam, Panniyur-1 and Karimunda were screened. In this study, seven rootstocks were evaluated for resistance based on the growth of pepper cuttings and resistance indexes to Meloidogyne incognita, which were surveyed 45 days after inoculation with M. incognita. The observation was recorded viz., nematode soil population (200 cc), number of galls, number of egg mass, number of females, gall index. All the Piper species show varying degree of response. Out of seven rootstocks used in this experiment IISR Sakthi were found to be highly resistant, Piper colubrinum, IISR Thevam and Karimunda resistant to root knot nematode and these cultivars can be used as a source of resistance. Black pepper (Piper nigrum L.), in particular, suffer extensive damage due to root-knot nematodes, and only a few wild species are known to be resistant. Grafting of cultivated plants to rootstocks of known resistant Piper species rootstocks could be an effective method to resolve this problem.
Cobb N A. 1918. Estimating the nematode population of soil. Agric. Tech.Circ.1: 1-48.
Eapen SJ, Beena B & Ramana K. 2005. Tropical soil microflora of spice-based cropping systems as potential antagonists of root-knot nematodes. Journal of Invertebrate Pathology, 88(3): 218-225.
Gordo SM, Pinheiro DG, Moreira EC, Rodrigues SM, Poltronieri MC, De Lemos OF & Schneider H. 2012. High-throughput sequencing of black pepper root transcriptome. BMC plant biology, 12(1): 168.
Haldhar SM, Choudhary BR, Bhargava R & Gurjar K. 2015. Host plant resistance (HPR) traits of ridge gourd (Luffa acutangula (Roxb.) L. against melon fruit fly, (Bactrocera cucurbitae (Coquillett)) in hot arid region of India. Scientia Horticulturae, 194, 168-174.
Khajuria A, Thusu N & Zutshi U. 2002. Piperine modulates permeability characteristics of intestine by inducing alterations in membrane dynamics: influence on brush border membrane fluidity, ultrastructure and enzyme kinetics. Phytomedicine, 9(3): 224-231.
Koshy PK & Sundaraju P. 1979. Response of seven black pepper cultivars to Meloidogyne incognita. Nematology Medit, 7: 123-248.
Nambiar K & Sarma Y. 1977. Wilt diseases of black pepper (India). Journal of plantation crops, 5: 92 - 103.
Ramana K & Mohandas C. 1987. Plant parasitic nematodes associated with black pepper (Piper nigrum L.) in Kerela. Indian Journal of Nematology, 17(1): 62-66.
Ramana KV & Mohandas C. 1989. Endoparasitic nematodes infesting roots at black pepper (Piper nigrum L.) in two districts at Karnataka, India. International Nematology Network Newsletter, 6: 33-55.
Sasser J, Powers H & Lucas G. 1957. Effect of root knot nematodes on the expression of black shank resistance in tobacco. Physiopath, 43: 483.
Thies JA & Fery RL. 1998. Modified expression of the N gene for southern root knot nematode resistance in pepper at high soil temperature. Journal of American Society for Horticulture Science, 123: 1012-1015.
Venkitesan T & Setty K. 1978. Reaction of 27 black pepper cultivars and wild forms to the burrowing nematode Radopholus similiscobb., Thorne (India). Journal of Plantation Crops, 7: 17-26.